Deformation measurement of individual cells in large populations using a single-cell microchamber array chip

Doh, I.; Lee, W. C.; Cho, Y. -H.; Pisano, A. P.; Kuypers, F. A.

doi:10.1063/1.4704923

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Deformation measurement of individual cells in large populations using a single-cell microchamber array chip

Doh, I.;
Lee, W. C.;
Cho, Y. -H.;
Pisano, A. P.;
Kuypers, F. A.

Citations

WEB OF SCIENCE

34

Citations

SCOPUS

43

초록

We analyze the deformability of individual red blood cells (RBCs) using SiCMA technology. Our approach is adequate to quickly measure large numbers of individual cells in heterogeneous populations. Individual cells are trapped in a large-scale array of micro-wells, and dielectrophoretic (DEP) force is applied to deform the cells. The simple structures of micro-wells and DEP electrodes facilitate the analysis of thousands of RBCs in parallel. This unique method allows the correlation of red cell deformation with cell surface and cytosolic characteristics to define the distribution of individual cellular characteristics in heterogeneous populations. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4704923]

키워드

Large-scale arrays; Microchamber array; Bioassay; Deformation; Cytosolic; Dielectrophoretic forces; Cytology; Red blood cell; Cell membranes; Red cells; Large population; Biochips; Deformation measurements; Heterogeneous populations; Simple structures; We; MECHANICAL-PROPERTIES; LASER-DIFFRACTION; DEFORMABILITY; EKTACYTOMETRY; PROTEIN; CANCER

제목: Deformation measurement of individual cells in large populations using a single-cell microchamber array chip

저자: Doh, I.; Lee, W. C.; Cho, Y. -H.; Pisano, A. P.; Kuypers, F. A.

DOI: 10.1063/1.4704923

발행일: 2012-04

유형: Article

저널명: Applied Physics Letters

권: 100

호: 17

언어: ENG

출판사: AMER INST PHYSICS

발행국가: 미국

ISSN: E 1077-3118
P 0003-6951