Levosulpiride-loaded nanostructured lipid carriers for brain delivery with antipsychotic and antidepressant effects

초록

Aims: Herein, we investigate the potential of levosulpiride-loaded nanostructured lipid carriers (LEVO-NLCs) for effective brain delivery with anti-psychotic and antidepressant effects. Main methods: Micro-emulsion method was used to prepare LEVO-NLCs, followed by its optimization using Design Expert (R), investigation of the particles properties and entrapment efficiency (%EE). Moreover, in-vitro release, in-vivo plasma and brain kinetic studies of LEVO-NLCs were executed. Anti-psychotic activity of LEVO-NLCs was accomplished in LPS-induced psychosis mice model. Additionally, expressions of neuro inflammatory mediators, neurodegeneration and neuro-inflammation in brain tissues was investigated. Key findings: The optimized LEVO-NLCs were rounded shaped nanoparticles (157.2 nm) with suitable zeta potential (-29.6 mV), low PDI (0.395) and high EE (83.67 %). No chemical interactions were found, however, the crystalline drug was changed to amorphous. LEVO-NLCs displayed sustained drug release behavior when compared with drug suspension. Moreover, a meaningfully higher AUC (106,642.27 +/- 876.44 ng.h/mL) and Cmax (38,534.72 +/- 2344.10 ng/mL) of the LEVO-NLCs in brain was observed as compared to the AUC (15,684.33 +/- 1005.49 ng.h/mL) and Cmax (7717.56 +/- 871.23 ng/mL) of LEVO-Suspension. Similar profiles of both the formulations were perceived in plasma pharmacokinetic studies. Furthermore, LEVO-NLCs exhibited a meaningfully improved anti-psychotic activity in LPS-induced psychosis mice model with reduced immobility time and enhanced struggling time. Likewise, treatment with LEVO-NLCs showed reduced levels of neuro inflammatory markers (p-NF-kappa B and COX-2) in LPS-induced mice. Additionally, no neuro-degeneration and neuroinflammation in brain tissues treated with LEVO-NLCs mice group was detected. Significance: These results concluded that NLCs may effectively be used for the brain delivery of various active pharmaceutical agents with enhanced biopharmaceutical performance.

키워드