Nanoplasmonic Rapid Antimicrobial-Resistance Point-of-Care Identification Device: RAPIDx

초록

The emergence of antibiotic resistance has become a global health crisis, andeveryone must arm themselves with wisdom to effectively combat the “silenttsunami” of infections that are no longer treatable with antibiotics. However,the overuse or inappropriate use of unnecessary antibiotics is still routine foradministering them due to the unavailability of rapid, precise, andpoint-of-care assays. Here, a rapid antimicrobial-resistance point-of-careidentification device (RAPIDx) is reported for the accurate and simultaneousidentification of bacterial species (genotype) and target enzyme activity(phenotype). First, a contamination-free active target enzyme is extracted viathe photothermal lysis of preconcentrated bacteria cells on a nanoplasmonicfunctional layer on-chip. Second, the rapid, precise identification of pathogensis achieved by the photonic rolling circle amplification of DNA on a chip.Third, the simultaneous identification of bacterial species (genotype) andtarget enzyme activity (phenotype) is demonstrated within a sample-to-answer45 min operation via the RAPIDx. It is believed that the RAPIDx will be avaluable method for solving the bottleneck of employing on-chipnanotechnology for antibiotic-resistant bioassay and other infectious diseases.

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